This lab has played a key role in working up this differential, as part of a "Long March" from the DNA in the nucleus to the ribosomes in the cytosol.
1. Brain ATP levels return to near-normal levels during early reperfusion, as shown by work published in the late 80s.
2. In 1991 and 1992, this lab published work demonstrating that brain nuclear and mitochondrial DNA is undamaged during early reperfusion.
3. Papers from other labs made it clear in the early 90's that the transcriptional machinery was intact.
4. This lab published work in the early 90s demonstrating that mRNA makes it out of the brain intact.
5. This lab also published work that "washed" or purified ribosomes isolated from brains after an ischemic insult could still translate mRNA to protein in an in vitro system.
Every emergency physician has had this experience: I know he's sick, but I can't find anything wrong! ATP
is cool, DNA is intact, transcription works, the mRNA wriggles out of
the nuke all right, and if I take out his ribosomes and clean 'em off, they work just fine. Maybe the guy just wants a work excuse.
Wait. What about dirty ribosomes?